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Development of olfactory abilities in children
Pípalová, Aneta ; Třebická Fialová, Jitka (advisor) ; Stopková, Romana (referee)
There is some interindividual variability in olfactory abilities and awareness of smells among humans, which can also be observed in children. Previous studies have shown a variety of factors that affects olfactory abilities, awareness and their development such as culture, diversity of olfactory environment, age, gender, verbal fluency or temperament. Olfactory abilities are measured using various psychophysical tools (identification, discrimination, sensitivity), and olfactory awareness is measured by metacognitive tests (e.g. the COBEL questionnaire on children's use of smell) that are adapted to children's cognitive and linguistic abilities. In this bachelor thesis, I summarize the available literature on the development and differences in children's olfactory abilities and awareness and the factors that affect them. Keywords: olfactory development in children, olfactory abilities, odour awareness, interindividual variability
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Chromosomal damage and DNA repair capacity in blood lymphocytes as transient markers in carcinogenesis.
Kroupa, Michal ; Vodička, Pavel (advisor) ; Štětina, Rudolf (referee)
Recent knowledge suggests that the onset of cancer is modulated by the interplay of internal and external environmental factors along with numerous gene variants. Structural chromsomal aberrations in peripheral blood lymphocytes are considered as biomarkers of effect of genotoxic carcinogens and reflect elevated risk of cancer. Incomplete or deficient repair of double-strand breaks in DNA underlie chromosomal aberrations and the measurement of cytogenetic alterations may reflect interindividual differences in the response towards the mutagen. In this study the expected deficiences in the DNA repair capacity have been determined in incident oncological patients with breast, colorectal and urogenital cancers. The determination of chromosomal aberrations have been supplemented by the measurement of variants in genes involved in double-strand breaks repair (XRCC3, rs861539; RAD54L, rs1048771). Methodologically, we employed conventional cytogenetic analysis, cytogenetic analysis following the induction of chromocomal damage by bleomycin ("Challenge assay"), TaqMan discrimination analysis for the detection of allelic variants and statistical analyses. By using these methods we did not observe statistically signifiant differences either in chromosomal breaks (p=0,354) or in a percentage of cells with...
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Reconstruction of the influence of grinding technology on upper limb muscle activity
Struška, Michal ; Sládek, Vladimír (advisor) ; Hora, Martin (referee)
Interpretation of changes of humeral asymmetry during the Holocene period was based on the analysis of upper limb muscle activity during cereal grinding using the saddle quern and rotary quern. The aim of the diploma thesis was to test if the dominant upper limb muscle activity during cereal grinding using the saddle quern and rotary quern might be estimated by measuring the activity of musculus deltoideus (pars clavicularis), musculus infraspinatus, musculus pectoralis major and musculus triceps brachii (caput longum). Using surface electromyography, we have analyzed activity of musculus biceps brachii, musculus deltoideus (pars clavicularis), musculus deltoideus (pars acromialis), musculus deltoideus (pars spinalis), musculus pectoralis major, musculus infraspinatus, musculus triceps brachii (caput laterale) and musculus triceps brachii (caput longum) during cereal grinding using the saddle quern and rotary quern in 25 subjects. Consistent with our prediction, musculus biceps brachii was the least active muscle during saddle quern grinding and clockwise rotary grinding, therefore it is possible to exclude musculus biceps brachii from the sample of analyzed muscles. Pars clavicularis, pars acromialis and pars spinalis of musculus deltoideus were more active during rotary quern grinding than...
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Chromosomal damage and DNA repair capacity in blood lymphocytes as transient markers in carcinogenesis.
Kroupa, Michal ; Vodička, Pavel (advisor) ; Štětina, Rudolf (referee)
Recent knowledge suggests that the onset of cancer is modulated by the interplay of internal and external environmental factors along with numerous gene variants. Structural chromsomal aberrations in peripheral blood lymphocytes are considered as biomarkers of effect of genotoxic carcinogens and reflect elevated risk of cancer. Incomplete or deficient repair of double-strand breaks in DNA underlie chromosomal aberrations and the measurement of cytogenetic alterations may reflect interindividual differences in the response towards the mutagen. In this study the expected deficiences in the DNA repair capacity have been determined in incident oncological patients with breast, colorectal and urogenital cancers. The determination of chromosomal aberrations have been supplemented by the measurement of variants in genes involved in double-strand breaks repair (XRCC3, rs861539; RAD54L, rs1048771). Methodologically, we employed conventional cytogenetic analysis, cytogenetic analysis following the induction of chromocomal damage by bleomycin ("Challenge assay"), TaqMan discrimination analysis for the detection of allelic variants and statistical analyses. By using these methods we did not observe statistically signifiant differences either in chromosomal breaks (p=0,354) or in a percentage of cells with...
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